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The circulating NPM mut.A levels were determined by qRT-PCR. The 20 μl reaction mixture contained 2 μl of DNA, 1 μl (0.4 μM) of sequence specific primers and 10 μl of SYBR Premix Ex Taq (TaKaRa, Tokyo, Japan). The sequences of the NPM mut.A primers were as follows: forward 5'-AGGCTATTCAAGATCTCTGTCTGG-3', and reverse 5'-AAGTTCTCACTCTGCATTATAAAAAGGA-3'. qRT-PCR was performed on a Rotor-Gene 6000 Real-Time PCR instrument (Corbett Research, Sydney, Australia). The copies of NPM mut.A in each sample were determined using the standard curve. The mean NPM mut.A copy number was calculated from triplicate reactions. All qRT-PCR assays were performed in a blinded fashion, without knowledge of specimen identity.. shown that for small children,.
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of the chip provide means for sample metering and mixing, and a. proportion of people knowing about VPDs were much lower as only. Undergraduate junior and senior health science students [medical (MS), nursing (NS) and medical laboratory students (MLS)] were recruited from higher education settings in Thessaly, Greece. Dichotomous questions, previously used, were adopted to assess knowledge, attitudes and concerns towards organ donation, together with questions regarding the recent presumed consent legislation.
Undergraduate junior and senior health science students [medical (MS), nursing (NS) and medical laboratory students (MLS)] were recruited from higher education settings in Thessaly, Greece. Dichotomous questions, previously used, were adopted to assess knowledge, attitudes and concerns towards organ donation, together with questions regarding the recent presumed consent legislation..
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To date, the pathogenesis of DHF/DSS remained not well understood. A number of hypotheses have been forwarded and these include the pathogenic effects of highly virulent variants of DENV [5] and the involvement of exaggerated host immune response [6-8]. Higher risk of contracting DHF/DSS in individuals with previous exposure to DENV has been well-documented [9, 10]. Evidences supporting the importance of antibody-dependent enhancement (ADE) of infection and T cell 'original antigenic sin' in induction of severe dengue have been presented. Alteration in the cytokine and T helper (Th) cell responses in secondary DENV infection has been described in the pathogenesis of severe dengue [6, 7]..
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Hypertensive crises, divided depending on the presence of target organ damage (TOD), are associated with increased cardiovascular mortality and morbidity. Monocyte chemoattractant protein-1 (MCP-1) is responsible for the recruitment of monocytes to sites of vascular inflammation. The aim of this study was to evaluate the role of vascular inflammation in development of TOD.. [9,10]. In addition, functional analysis in tissues is challenging owing to the. dysmenorrhoea, menorrhagia and irregular. Studies of omega-3 supplementation in dialysis patients describe salutary effects on lipid profiles. However, study results have been inconsistent. The aim of this study was to evaluate the influence of omega-3 supplementation on serum lipids in chronic dialysis patients.. which prevents the leakage of H+ into the GI tract.. Studies were identified by searching PubMed and ISI Web of Science up to February 2015. For the continuous outcomes is it legal to buy modafinil online uk we calculated the weighted mean difference and 95% confidence interval. The statistical analysis was performed by RevMan 5.1 and Stata 12 software. Only case-control studies were included if they had data on serum UCH-L1 levels in TBI patients and healthy controls. Funnel plot and Egger's regression test were applied to assess the potential publication bias..
